An investigation of the effects of Cinnamomum cassia bark extracts on oxidative DNA damage and possible cytotoxic and apoptotic activities in transformed/untransformed cell lines from Type 1 diabetic patients, in vitro.
An investigation of the effects of Cinnamomum cassia bark extracts on oxidative DNA damage and possible cytotoxic and apoptotic activities in transformed/untransformed cell lines from Type 1 diabetic patients, in vitro.
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It was shown that patients with Type 1 diabetes mellitus (T1DM) had increased level of oxidative DNA damage and decreased efficacy of DNA repair.These changes were implicated in the increased cancer risk in patients with diabetes mellitus.Cinnamon bark extracts have diverse biological activities including antidiabetic and anti-tumor properties.Cinnamomum cassia (C.cassia) is a common used cinnamon species present in commercial cinnamon preparations.
We aimed to investigate the effects of cinnamon extracts prepared from C.cassia bark on endogenous and hydrogen peroxide (H2O2)-induced oxidative DNA damage, as well as cytotoxic PV Inverter and apoptotic activities in this study.Type 1 diabetic (T1DM) lymphocytes (GM02765, GM01838) and fibroblasts (GM01837) were obtained from NIGMS Human Genetic Cell Repository of Coriell Institute, New Jersey, USA.Cytotoxicity analysis were performed by using a tetrazolium salt, 4-[3-(4-iodophenyl) 2-(4-nitrophenyl) 2H-5-tetrazolio] 1,3-benzene disulfonate (WST-1).The effects of extracts on endogenous and H2O2-induced oxidative DNA damage were studied using the single cell gel electrophoresis (SCGE; Comet Assay), a technique allowing DNA damage in a single cell.
Apoptotic activities of extracts were investigated by TUNEL and Annexin V/PI assays.using flow cytometry.IC50 and IC20 values of the extracts varied and the effects on endogenous and H2O2-induced DNA damage were different regarding cell lines and extracts.Although their protective effects at some doses Serving Platter against to H2O2-induced oxidative damage, our results suggested DNA damaging and apoptotic potential of cinnamon bark extracts on Type 1 diabetic cell lines, in vitro.